Research Papers: Therapeutic

Single session of Nd:YAG laser intracanal irradiation neutralizes endotoxin in dental root dentin

[+] Author Affiliations
José R. F. Archilla, Maria S. N. A. Moreira, Antônio C. Bombana, Márcia M. Marques

Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brazil

Sueli P. H. Miyagi

Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brazil

Universidade Braz Cubas, Faculdade de Odontologia, Mogi das Cruzes, SP, Brazil

Norbert Gutknecht

Aachen Dental Laser Center at RWTH Aachen University, Templergraben 55, 52056 Aachen, Germany

J. Biomed. Opt. 17(11), 118002 (Nov 19, 2012). doi:10.1117/1.JBO.17.11.118002
History: Received July 30, 2012; Revised October 22, 2012; Accepted October 23, 2012
Text Size: A A A

Abstract.  Endotoxins released in the dental root by Gram-negative microorganisms can be neutralized by calcium hydroxide, when this medication is applied inside the root canal for at least seven days. However, several clinical situations demand faster root canal decontamination. Thus, for faster endotoxin neutralization, endodontists are seeking additional treatments. The in vitro study tested whether or not intracanal Nd:YAG laser irradiation would be able to neutralize endotoxin within the human dental root canal in a single session. Twenty-four human teeth with one root were mounted between two chambers. After conventional endodontic treatment, root canals were contaminated with Escherichia coli endotoxin. Then they were irradiated or not (controls) in contact mode with an Nd:YAG laser (1.5 W, 15 Hz, 100 mJ and pulse fluency of 124J/cm2). The endotoxin activity was measured using the limulus lysate technique and data were statistically compared (p0.05). The concentration of active endotoxin measured in the negative control group was significantly lower than that of the positive control group (p=0.04). The concentrations of endotoxin in both irradiated groups were significantly lower than that of the positive control group (p=0.027) and similar to that of negative control group (p=0.20). A single session of intracanal Nd:YAG laser irradiation is able to neutralize endotoxin in the dental root tissues.

© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

José R. F. Archilla ; Maria S. N. A. Moreira ; Sueli P. H. Miyagi ; Antônio C. Bombana ; Norbert Gutknecht, et al.
"Single session of Nd:YAG laser intracanal irradiation neutralizes endotoxin in dental root dentin", J. Biomed. Opt. 17(11), 118002 (Nov 19, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.118002


Figures

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.