Research Papers: Imaging

Indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy

[+] Author Affiliations
Kinshuk Mitra, James Melvin, Shufang Chang, Ronald X. Xu

Ohio State University, Department of Biomedical Engineering, Columbus, Ohio 43210

Kyoungjin Park, Alper Yilmaz

Ohio State University, Photogrammetric Computer Vision Lab, Columbus, Ohio 43210

Scott Melvin

Ohio State University, Department of Surgery, Columbus, Ohio 43210

J. Biomed. Opt. 17(11), 116025 (Nov 19, 2012). doi:10.1117/1.JBO.17.11.116025
History: Received July 26, 2012; Revised October 10, 2012; Accepted October 15, 2012
Text Size: A A A

Abstract.  We encapsulate indocyanine green (ICG) in poly[(D,L-lactide-co-glycolide)-co-PEG] diblock (PLGA-PEG) microballoons for real-time fluorescence and hyperspectral imaging of biliary anatomy. ICG-loaded microballoons show superior fluorescence characteristics and slower degradation in comparison with pure ICG. The use of ICG-loaded microballoons in biliary imaging is demonstrated in both biliary-simulating phantoms and an ex vivo tissue model. The biliary-simulating phantoms are prepared by embedding ICG-loaded microballoons in agar gel and imaged by a fluorescence imaging module in a Da Vinci surgical robot. The ex vivo model consists of liver, gallbladder, common bile duct, and part of the duodenum freshly dissected from a domestic swine. After ICG-loaded microballoons are injected into the gallbladder, the biliary structure is imaged by both hyperspectral and fluorescence imaging modalities. Advanced spectral analysis and image processing algorithms are developed to classify the tissue types and identify the biliary anatomy. While fluorescence imaging provides dynamic information of movement and flow in the surgical region of interest, data from hyperspectral imaging allow for rapid identification of the bile duct and safe exclusion of any contaminant fluorescence from tissue not part of the biliary anatomy. Our experiments demonstrate the technical feasibility of using ICG-loaded microballoons for biliary imaging in cholecystectomy.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers


Kinshuk Mitra ; James Melvin ; Shufang Chang ; Kyoungjin Park ; Alper Yilmaz, et al.
"Indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy", J. Biomed. Opt. 17(11), 116025 (Nov 19, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.116025

Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content


Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections


Buy this article ($18 for members, $25 for non-members).
Sign In