Special Section on Optical Methods of Imaging in the Skin

Advanced chemical imaging and comparison of human and porcine hair follicles for drug delivery by confocal Raman microscopy

[+] Author Affiliations
Lutz Franzen, Christiane Mathes

Saarland University, Department of Biopharmaceutics and Pharmaceutical Technology, Saarbruecken, Germany

Steffi Hansen

Saarland University, Department of Biopharmaceutics and Pharmaceutical Technology, Saarbruecken, Germany

Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Center for Infection Research, Saarbruecken, Germany

Maike Windbergs

Saarland University, Department of Biopharmaceutics and Pharmaceutical Technology, Saarbruecken, Germany

Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Center for Infection Research, Saarbruecken, Germany

PharmBioTec GmbH, Saarbruecken, Germany

J. Biomed. Opt. 18(6), 061210 (Nov 19, 2012). doi:10.1117/1.JBO.18.6.061210
History: Received September 11, 2012; Revised October 22, 2012; Accepted October 23, 2012
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Abstract.  Hair follicles have recently gained a lot of interest for dermal drug delivery. They provide facilitated penetration into the skin and a high potential to serve as a drug depot. In this area of research, excised pig ear is a widely accepted in vitro model to evaluate penetration of drug delivery into hair follicles. However, a comparison of human and porcine follicles in terms of chemical composition has not been performed so far. In this study, we applied confocal Raman microscopy as a chemically selective imaging technique to compare human and porcine follicle composition and to visualize component distribution within follicle cross-sections. Based on the evaluation of human and porcine Raman spectra optical similarity for both species was successfully confirmed. Furthermore, cyanoacrylate skin surface biopsies, which are generally used to determine the extent of follicular penetration, were imaged by a novel complementary analytical approach combining confocal Raman microscopy and optical profilometry. This all-encompassing analysis allows investigation of intactness and component distribution of the excised hair bulb in three dimensions. Confocal Raman microscopy shows a high potential as a noninvasive and chemically selective technique for the analysis of trans-follicular drug delivery.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Lutz Franzen ; Christiane Mathes ; Steffi Hansen and Maike Windbergs
"Advanced chemical imaging and comparison of human and porcine hair follicles for drug delivery by confocal Raman microscopy", J. Biomed. Opt. 18(6), 061210 (Nov 19, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.6.061210


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