Special Section on Multiphoton Microscopy: Technical Innovations, Biological Applications, and Clinical Diagnostics

Study of molecule variations in renal tumor based on confocal micro-Raman spectroscopy

[+] Author Affiliations
Zhengfei Zhuang, Ning Li, Zhouyi Guo, Meifang Zhu

South China Normal University, College of Biophotonics, MOE Key Laboratory of Laser Life Science, Guangzhou 510631, Guangdong, China

Ke Xiong, Sijin Chen

Southern Medical University, Nanfang Hospital, Guangzhou 510515, Guangdong, China

J. Biomed. Opt. 18(3), 031103 (Nov 22, 2012). doi:10.1117/1.JBO.18.3.031103
History: Received May 23, 2012; Revised August 11, 2012; Accepted August 15, 2012
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Abstract.  Confocal micro-Raman spectroscopy—a valuable analytical tool in biological and medical field of research—allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman peaks of the renal tumor at 788 and 1087cm1, which belong to νsPO2 and νasPO2 stretching, respectively, have an obvious increase. At the same time, the ratio of I855/I831 in renal tumor tissue is 1.39±0.08, while that in normal renal tissue is 2.44±0.05 (p<0.01). This means that more tyrosine conformation transform from “buried” to “exposed” in the presence of cancer. Principal component analysis is used to classify the Raman spectra of renal tumor tissue and normal tissue.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Zhengfei Zhuang ; Ning Li ; Zhouyi Guo ; Meifang Zhu ; Ke Xiong, et al.
"Study of molecule variations in renal tumor based on confocal micro-Raman spectroscopy", J. Biomed. Opt. 18(3), 031103 (Nov 22, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.3.031103


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