Special Section on Optical Methods of Imaging in the Skin

Changes in the redox state and endogenous fluorescence of in vivo human skin due to intrinsic and photo-aging, measured by multiphoton tomography with fluorescence lifetime imaging

[+] Author Affiliations
Washington Y. Sanchez, Elizabeth Ryan, Jeffrey E. Grice

University of Queensland, Therapeutics Research Centre, School of Medicine, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia

Clara Obispo

Institut Polytechnique Lasalle-Beauvais, Department of Nutrition and Health Sciences, Beauvais, Picardie, France

Michael S. Roberts

University of Queensland, Therapeutics Research Centre, School of Medicine, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia

University of South Australia, School of Pharmacy and Medical Science, City East Campus, Adelaide, South Australia, Australia

J. Biomed. Opt. 18(6), 061217 (Nov 27, 2012). doi:10.1117/1.JBO.18.6.061217
History: Received September 18, 2012; Revised November 2, 2012; Accepted November 2, 2012
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Abstract.  Ultraviolet radiation from solar exposure is a key extrinsic factor responsible for premature skin aging (i.e., photo-aging). Recent advances using in vivo multiphoton tomography (MPT) demonstrate the efficacy of this approach to assess intrinsic and extrinsic skin aging as an alternative to existing invasive techniques. In this study, we measured changes in epidermal autofluorescence, dermal collagen second harmonic generation (SHG), and the redox state of solar-exposed and solar-protected human skin by MPT with fluorescence lifetime imaging (MPT-FLIM). Twenty-four volunteers across four age categories (20 to 29, 30 to 39, 40 to 49, and 50 to 59 years old; six volunteers each) were recruited for MPT-FLIM imaging of the dorsal (solar-exposed; photo-damaged) and volar (solar-protected) forearm. We demonstrate a higher intensity of dermal collagen SHG within the volar forearm compared to dorsal solar-exposed skin. Redox imaging of each epidermal skin stratum by FLIM demonstrates an increase in fluorescence lifetime in the solar-exposed dorsal forearm that is more apparent in aged skin. The results of this study suggest the redox state of the viable epidermis is a key marker in assessing intrinsic and photo-damage skin aging, in combination with changes in autofluorescence and SHG.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Washington Y. Sanchez ; Clara Obispo ; Elizabeth Ryan ; Jeffrey E. Grice and Michael S. Roberts
"Changes in the redox state and endogenous fluorescence of in vivo human skin due to intrinsic and photo-aging, measured by multiphoton tomography with fluorescence lifetime imaging", J. Biomed. Opt. 18(6), 061217 (Nov 27, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.6.061217


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