Special Section on Optical Methods of Imaging in the Skin

Structured illumination microscopy using random intensity incoherent reflectance

[+] Author Affiliations
Zachary R. Hoffman

Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts 02115

Raytheon BBN Technologies, 10 Moulton Street, Cambridge, Massachusetts 02138

Charles A. DiMarzio

Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts 02115

Northeastern University, Department of Mechanical and Industrial Engineering, Boston, Massachusetts 02115

J. Biomed. Opt. 18(6), 061216 (Nov 27, 2012). doi:10.1117/1.JBO.18.6.061216
History: Received September 8, 2012; Revised October 30, 2012; Accepted November 1, 2012
Text Size: A A A

Abstract.  Depth information is resolved from thick specimens using a modification of structured illumination. By projecting a random projection pattern with varied spatial frequencies that is rotated while capturing images, sectioning can be performed using an incoherent light source in reflectance only. This provides a low-cost solution to obtaining information similar to that produced in confocal microscopy and other methods of structured illumination, without the requirement of complex or elaborate equipment, coherent light sources, or fluorescence. The broad line width of the light emitting diode minimizes artifacts associated with speckle from the laser while also increasing the safety of the instrument. Single diffusers and cascaded diffusers are compared to provide the most efficient method for sectioning at depth. By using reflectance only, in vivo images are produced on a human subject, generating high-contrast images and providing depth information about subsurface objects.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Zachary R. Hoffman and Charles A. DiMarzio
"Structured illumination microscopy using random intensity incoherent reflectance", J. Biomed. Opt. 18(6), 061216 (Nov 27, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.6.061216


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.