Research Papers: Imaging

Investigation of lipid homeostasis in living Drosophila by coherent anti-Stokes Raman scattering microscopy

[+] Author Affiliations
Cheng-Hao Chien

National Yang-Ming University, Institute of Biophotonics, Taipei 112, Taiwan

Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan

Wei-Wen Chen

Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan

Institute of Atomic and Molecular Sciences, Molecular Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei 106, Taiwan

National Tsing Hua University, Department of Chemistry, Hsinchu 30013, Taiwan

June-Tai Wu

National Taiwan University, Institute of Molecular Medicine, College of Medicine, Taipei 100, Taiwan

National Taiwan University Hospital, Department of Medical Research, Taipei 100, Taiwan

Ta-Chau Chang

National Yang-Ming University, Institute of Biophotonics, Taipei 112, Taiwan

Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan

Institute of Atomic and Molecular Sciences, Molecular Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei 106, Taiwan

J. Biomed. Opt. 17(12), 126001 (Dec 03, 2012). doi:10.1117/1.JBO.17.12.126001
History: Received September 21, 2012; Revised November 9, 2012; Accepted November 12, 2012
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Abstract.  To improve our understanding of lipid metabolism, Drosophila is used as a model animal, and its lipid homeostasis is monitored by coherent anti-Stokes Raman scattering microscopy. We are able to achieve in vivo imaging of larval fat body (analogous to adipose tissue in mammals) and oenocytes (analogous to hepatocytes) in Drosophila larvae at subcellular level without any labeling. By overexpressing two lipid regulatory proteins—Brummer lipase (Bmm) and lipid storage droplet-2 (Lsd-2)—we found different phenotypes and responses under fed and starved conditions. Comparing with the control larva, we observed more lipid droplet accumulation by twofold in oenocytes of fat-body-Bmm-overexpressing (FB-Bmm-overexpressing) mutant under fed condition, and less lipid by fourfold in oenocytes of fat-body-Lsd-2-overexpressing (FB-Lsd-2-overexpressing) mutant under starved condition. Moreover, together with reduced size of lipid droplets, the lipid content in the fat body of FB-Bmm-overexpressing mutant decreases much faster than that of the control and FB-Lsd-2-overexpressing mutant during starvation. From long-term starvation assay, we found FB-Bmm-overexpressing mutant has a shorter lifespan, which can be attributed to faster consumption of lipid in its fat body. Our results demonstrate in vivo observations of direct influences of Bmm and Lsd-2 on lipid homeostasis in Drosophila larvae.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Cheng-Hao Chien ; Wei-Wen Chen ; June-Tai Wu and Ta-Chau Chang
"Investigation of lipid homeostasis in living Drosophila by coherent anti-Stokes Raman scattering microscopy", J. Biomed. Opt. 17(12), 126001 (Dec 03, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.12.126001


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