Special Section on Optical Methods of Imaging in the Skin

Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data

[+] Author Affiliations
Hagar I. Labouta

Helmholtz Center for Infection Research, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany

University of Alexandria, Department of Pharmaceutics, Alexandria, Egypt

Sibylle Thude

Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Stuttgart, Germany

Marc Schneider

Saarland University, Department of Pharmaceutical Nanotechnology, Saarbrücken, Germany

J. Biomed. Opt. 18(6), 061218 (Nov 30, 2012). doi:10.1117/1.JBO.18.6.061218
History: Received August 30, 2012; Revised November 7, 2012; Accepted November 7, 2012
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Abstract.  Owing to the limited source of human skin (HS) and the ethical restrictions of using animals in experiments, in vitro skin equivalents are a possible alternative for conducting particle penetration experiments. The conditions for conducting penetration experiments with model particles, 15-nm gold nanoparticles (AuNP), through nonsealed skin equivalents are described for the first time. These conditions include experimental setup, sterility conditions, effective applied dose determination, skin sectioning, and skin integrity check. Penetration at different exposure times (two and 24 h) and after tissue fixation (fixed versus unfixed skin) are examined to establish a benchmark in comparison to HS in an attempt to get similar results to HS experiments presented earlier. Multiphoton microscopy is used to detect gold luminescence in skin sections. λex=800nm is used for excitation of AuNP and skin samples, allowing us to determine a relative index for particle penetration. Despite the observed overpredictability of penetration into skin equivalents, they could serve as a first fast screen for testing the behavior of nanoparticles and extrapolate their penetration behavior into HS. Further investigations are required to test a wide range of particles of different physicochemical properties to validate the skin equivalent-human skin particle penetration relationship.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Hagar I. Labouta ; Sibylle Thude and Marc Schneider
"Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data", J. Biomed. Opt. 18(6), 061218 (Nov 30, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.6.061218


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