Research Papers: Imaging

Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy

[+] Author Affiliations
Peter Dedecker

The Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 725 N. Wolfe Street, Baltimore, Mayland 21205

Katholieke Universiteit Leuven, Department of Chemistry, Celestijnenlaan 200F, 3001 Heverlee, Belgium

Sam Duwé, Robert K. Neely

Katholieke Universiteit Leuven, Department of Chemistry, Celestijnenlaan 200F, 3001 Heverlee, Belgium

Jin Zhang

The Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 725 N. Wolfe Street, Baltimore, Mayland 21205

J. Biomed. Opt. 17(12), 126008 (Dec 03, 2012). doi:10.1117/1.JBO.17.12.126008
History: Received July 5, 2012; Revised October 31, 2012; Accepted October 31, 2012
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Abstract.  We present Localizer, a freely available and open source software package that implements the computational data processing inherent to several types of superresolution fluorescence imaging, such as localization (PALM/STORM/GSDIM) and fluctuation imaging (SOFI/pcSOFI). Localizer delivers high accuracy and performance and comes with a fully featured and easy-to-use graphical user interface but is also designed to be integrated in higher-level analysis environments. Due to its modular design, Localizer can be readily extended with new algorithms as they become available, while maintaining the same interface and performance. We provide front-ends for running Localizer from Igor Pro, Matlab, or as a stand-alone program. We show that Localizer performs favorably when compared with two existing superresolution packages, and to our knowledge is the only freely available implementation of SOFI/pcSOFI microscopy. By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Peter Dedecker ; Sam Duwé ; Robert K. Neely and Jin Zhang
"Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy", J. Biomed. Opt. 17(12), 126008 (Dec 03, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.12.126008


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