Research Papers: Imaging

Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents

[+] Author Affiliations
Pierre-Yves Fortin, Coralie Genevois

Université Bordeaux Segalen, Laboratoire IMF, CNRS/UMR 5231, Université Bordeaux 2, France

Université Bordeaux Segalen, Institut de BioImagerie, CNRS/UMS 3428, Université Bordeaux 2, France

Anne Koenig, Emilie Heinrich, Isabelle Texier

CEA/LETI Minatec, Grenoble, France

Franck Couillaud

Université Bordeaux Segalen, Laboratoire IMF, CNRS/UMR 5231, Université Bordeaux 2, France

Université Bordeaux Segalen, Laboratoire RMSB, CNRS/UMR 5536, Université Bordeaux 2, France

J. Biomed. Opt. 17(12), 126004 (Dec 03, 2012). doi:10.1117/1.JBO.17.12.126004
History: Received June 1, 2012; Revised October 29, 2012; Accepted October 30, 2012
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Abstract.  Near-infrared fluorescence-enhanced diffuse optical tomography (fDOT) is used to localize tumors in mice using fluorescent nanoparticles as a blood pool contrast agent. The infrared dye DiR is loaded in the lipid core of nontargeted nanoparticles (DiR-lipidots) and injected systemically via the tail vein in mice bearing U87 tumors. Distribution and time-course of DiR-lipidots are followed using in vivo fluorescence reflectance imaging and reveal enhanced fluorescent signal within the subcutaneous tumors up to seven days due to the enhanced permeability and retention effect. Tumor growth into the brain is followed using bioluminescent imaging, and tumor localization is further determined by magnetic resonance imaging. The fDOT provides three-dimensional fluorescent maps that allow for consistent localization for both subcutaneous and brain tumors.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Pierre-Yves Fortin ; Coralie Genevois ; Anne Koenig ; Emilie Heinrich ; Isabelle Texier, et al.
"Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents", J. Biomed. Opt. 17(12), 126004 (Dec 03, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.12.126004


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