JBO Letters

Objective-free optical-resolution photoacoustic microscopy

[+] Author Affiliations
Chulhong Kim, Mansik Jeon

The State University of New York, The University at Buffalo, Bio Optics and Acoustics Laboratory, Department of Biomedical Engineering, Buffalo, New York 14260

Sungjo Park, Changho Lee, Jeehyun Kim

Kyungpook National University, School of Electrical Engineering and Computer Science, Daegu, Republic of Korea

Jongki Kim, Sungrae Lee, Kyunghwan Oh

Yonsei University, Institute of Physics and Applied Physics, Department of Physics, Photonic Device Physics Laboratory, Seoul, Republic of Korea

J. Biomed. Opt. 18(1), 010501 (Dec 11, 2012). doi:10.1117/1.JBO.18.1.010501
History: Received August 14, 2012; Revised November 12, 2012; Accepted November 26, 2012
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Abstract.  Optical-resolution photoacoustic microscopy (OR-PAM) becomes a premier microscopic imaging tool in biomedicine because it provides agent-free optical absorption information in tissues. By tightly focusing light to a spot, a significantly improved lateral resolution can be achieved in OR-PAM. The focal spot size is typically determined by the numerical aperture of the used objective lens. Here, we demonstrate objective-free OR-PAM using a fiber optic Bessel beam generator. In this approach, no objective lens is required and, beneficially, the complexities of conventional OR-PAM systems can be greatly relieved. We have obtained photoacoustic images of a carbon fiber with a diameter of 6μm, whose lateral resolution was measured to be better than 6 to 7 μm.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Chulhong Kim ; Sungjo Park ; Jongki Kim ; Sungrae Lee ; Changho Lee, et al.
"Objective-free optical-resolution photoacoustic microscopy", J. Biomed. Opt. 18(1), 010501 (Dec 11, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.1.010501


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