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Research Papers: Imaging

Longitudinal in vivo monitoring of rodent glioma models through thinned skull using laser speckle contrast imaging

[+] Author Affiliations
Abhishek Rege

Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland

Alan C. Seifert, Dan Schlattman, Yu Ouyang

Infinite Biomedical Technologies LLC, Baltimore, Maryland

Khan W. Li, Luca Basaldella, Betty M. Tyler

Johns Hopkins University, Department of Neurosurgery, Baltimore, Maryland

Henry Brem

Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland

Johns Hopkins University, Department of Neurosurgery, Baltimore, Maryland

Johns Hopkins University, Departments of Oncology and Ophthalmology, Baltimore, Maryland

Nitish V. Thakor

Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland

Infinite Biomedical Technologies LLC, Baltimore, Maryland

J. Biomed. Opt. 17(12), 126017 (Dec 12, 2012). doi:10.1117/1.JBO.17.12.126017
History: Received August 7, 2012; Revised November 8, 2012; Accepted November 9, 2012
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Abstract.  Laser speckle contrast imaging (LSCI) is a contrast agent free imaging technique suited for longitudinal assessment of vascular remodeling that accompanies brain tumor growth. We report the use of LSCI to monitor vascular changes in a rodent glioma model. Ten rats are inoculated with 9L gliosarcoma cells, and the angiogenic response is monitored five times over two weeks through a thinned skull imaging window. We are able to visualize neovascularization and measure the number of vessels per unit area to assess quantitatively the microvessel density (MVD). Spatial spread of MVD reveals regions of high MVD that may correspond to tumor location. Whole-field average MVD values increase with time in the tumor group but are fairly stable in the control groups. Statistical analysis shows significant differences in MVD values between the tumor group and both saline-receiving and unperturbed control groups over the two-week period (p<0.05). In conclusion, LSCI is suitable for investigation of tumor angiogenesis in rodent models. In addition, the statistical difference (p<0.02) between MVD values of the tumor (24.40±1.41) and control groups (15.40±1.60) on the 14th day after inoculation suggests a potential use of LSCI in the clinic in distinguishing tumor environments from normal vasculature.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Topics

Brain ; Lasers ; Skull ; Speckle

Citation

Abhishek Rege ; Alan C. Seifert ; Dan Schlattman ; Yu Ouyang ; Khan W. Li, et al.
"Longitudinal in vivo monitoring of rodent glioma models through thinned skull using laser speckle contrast imaging", J. Biomed. Opt. 17(12), 126017 (Dec 12, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.12.126017


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