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Research Papers: Therapeutic

Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: an in vitro study of macrophages as a vector for the delivery of gold nanoshells

[+] Author Affiliations
Taeseok Daniel Yang, Wonshik Choi, Tai Hyun Yoon, Kyoung Jin Lee

Korea University, Department of Physics, Seoul, Republic of Korea

Jae-Seung Lee, Sang Hun Han

Korea University, Department of Materials Science and Engineering, Seoul, Republic of Korea

Min-Goo Lee, Hong Soon Yim, Kyung Min Choi

Korea University, Department of Physiology, Seoul, Republic of Korea

Min Woo Park, Kwang-Yoon Jung, Seung-Kuk Baek

Korea University, Department of Otolaryngology-Head and Neck Surgery, Seoul, Republic of Korea

J. Biomed. Opt. 17(12), 128003 (Dec 12, 2012). doi:10.1117/1.JBO.17.12.128003
History: Received September 26, 2012; Revised November 8, 2012; Accepted November 21, 2012
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Abstract.  Photothermal treatment (PTT) using nanoparticles has gained attention as a promising alternative therapy for malignant tumors. One strategy for increasing the selectivity of PTT is the use of macrophages as a cellular vector for delivering nanoparticles. The aim of the present study is to examine the use of macrophages as a cellular vector for efficient PTT and determine the appropriate irradiation power and time of a near-infrared (NIR) laser using real-time phase-contrast imaging. Thermally induced injury and death of cancer cells were found to begin at 44°C to 45°C, which was achieved using the PTT effect with gold nanoshells (NS) and irradiation with a NIR laser at a power of 2 W for 5 min. The peritoneal macrophage efficiently functioned as a cellular vector for the NS, and the cancer cells surrounding the NS-loaded macrophages selectively lost their cellular viability after being irradiated with the NIR laser.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Taeseok Daniel Yang ; Wonshik Choi ; Tai Hyun Yoon ; Kyoung Jin Lee ; Jae-Seung Lee, et al.
"Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: an in vitro study of macrophages as a vector for the delivery of gold nanoshells", J. Biomed. Opt. 17(12), 128003 (Dec 12, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.12.128003


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