Research Papers: Imaging

Photoacoustic microscopy of bilirubin in tissue phantoms

[+] Author Affiliations
Yong Zhou, Chi Zhang, Da-Kang Yao, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 17(12), 126019 (Dec 13, 2012). doi:10.1117/1.JBO.17.12.126019
History: Received April 11, 2012; Revised November 19, 2012; Accepted November 20, 2012
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Abstract.  Determining both bilirubin’s concentration and its spatial distribution are important in disease diagnosis. Here, for the first time, we applied quantitative multiwavelength photoacoustic microscopy (PAM) to detect bilirubin concentration and distribution simultaneously. By measuring tissue-mimicking phantoms with different bilirubin concentrations, we showed that the root-mean-square error of prediction has reached 0.52 and 0.83mg/dL for pure bilirubin and for blood-mixed bilirubin detection (with 100% oxygen saturation), respectively. We further demonstrated the capability of the PAM system to image bilirubin distribution both with and without blood. Finally, by underlaying bilirubin phantoms with mouse skins, we showed that bilirubin can be imaged with consistent accuracy down to >400μm in depth. Our results show that PAM has potential for noninvasive bilirubin monitoring in vivo, as well as for further clinical applications.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Yong Zhou ; Chi Zhang ; Da-Kang Yao and Lihong V. Wang
"Photoacoustic microscopy of bilirubin in tissue phantoms", J. Biomed. Opt. 17(12), 126019 (Dec 13, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.12.126019


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