Research Papers: Imaging

Ratio images and ultraviolet C excitation in autofluorescence imaging of neoplasms of the human colon

[+] Author Affiliations
Timothy E. Renkoski, Logan R. Graves

University of Arizona, College of Optical Sciences, 1630 E. University Boulevard, Tucson, Arizona 85721

Bhaskar Banerjee

University of Arizona, College of Optical Sciences, 1630 E. University Boulevard, Tucson, Arizona 85721

University of Arizona, Department of Medicine, Section of Gastroenterology, 1501 N. Campbell Avenue, P.O. Box 245028, Tucson, Arizona 85724-5028

University of Arizona, Department of Biomedical Engineering, 1127 E. James E. Rogers Way, Tucson, Arizona 85721

Nathaniel S. Rial, Sirandon A. H. Reid, Piyush Tiwari, Hemanth Gavini

University of Arizona, Department of Medicine, Section of Gastroenterology, 1501 N. Campbell Avenue, P.O. Box 245028, Tucson, Arizona 85724-5028

Vassiliki Liana Tsikitis, Valentine N. Nfonsam

University of Arizona, Department of Surgery, 1501 N. Campbell Avenue, Tucson, Arizona 85724

Urs Utzinger

University of Arizona, College of Optical Sciences, 1630 E. University Boulevard, Tucson, Arizona 85721

University of Arizona, Department of Biomedical Engineering, 1127 E. James E. Rogers Way, Tucson, Arizona 85721

J. Biomed. Opt. 18(1), 016005 (Jan 04, 2013). doi:10.1117/1.JBO.18.1.016005
History: Received July 17, 2012; Revised November 14, 2012; Accepted December 3, 2012
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Abstract.  The accepted screening technique for colon cancer is white light endoscopy. While most abnormal growths (lesions) are detected by this method, a significant number are missed during colonoscopy, potentially resulting in advanced disease. Missed lesions are often flat and inconspicuous in color. A prototype ultraviolet spectral imager measuring autofluorescence (AF) and reflectance has been developed and applied in a study of 21 fresh human colon surgical specimens. Six excitation wavelengths from 280 to 440 nm and formulaic ratio imaging were utilized to increase lesion contrast and cause neoplasms to appear bright compared to normal tissue. It was found that in the subset of lesions which were most difficult to visualize in standard color photographs [low contrast lesions, (LCLs)] a ratio image (F340/F440) of AF images excited at 340 and 440 nm produced extraordinary images and was effective in about 70% of these difficult cases. Contrast may be due to increased levels of reduced nicotinamide adenine dinucleotide, increased hemoglobin absorption, and reduced signal from submucosal collagen. A second successful ratio image (R480/R555) combined two reflectance images to produce exceptional images especially in particular LCLs where F340/F440 was ineffective. The newly discovered ratio images can potentially improve detection rate in screening with a novel AF colonoscope.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Timothy E. Renkoski ; Bhaskar Banerjee ; Logan R. Graves ; Nathaniel S. Rial ; Sirandon A. H. Reid, et al.
"Ratio images and ultraviolet C excitation in autofluorescence imaging of neoplasms of the human colon", J. Biomed. Opt. 18(1), 016005 (Jan 04, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.1.016005


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