Special Section on Optical Methods of Imaging in the Skin

Stepwise multiphoton activation fluorescence reveals a new method of melanin detection

[+] Author Affiliations
Zhenhua Lai, Josef Kerimo, Yair Mega

Northeastern University, Electrical and Computer Engineering Department, Boston, Massachusetts 02115

Northeastern University, Bernard M. Gordon Center for Subsurface Sensing and Imaging System, Boston, Massachusetts 02115

Charles A. DiMarzio

Northeastern University, Electrical and Computer Engineering Department, Boston, Massachusetts 02115

Northeastern University, Mechanical Engineering Department, Boston, Massachusetts 02115

Northeastern University, Bernard M. Gordon Center for Subsurface Sensing and Imaging System, Boston, Massachusetts 02115

J. Biomed. Opt. 18(6), 061225 (Jan 07, 2013). doi:10.1117/1.JBO.18.6.061225
History: Received September 10, 2012; Revised December 1, 2012; Accepted December 5, 2012
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Abstract.  The stepwise multiphoton activated fluorescence (SMPAF) of melanin, activated by a continuous-wave mode near infrared (NIR) laser, reveals a broad spectrum extending from the visible spectra to the NIR and has potential application for a low-cost, reliable method of detecting melanin. SMPAF images of melanin in mouse hair and skin are compared with conventional multiphoton fluorescence microscopy and confocal reflectance microscopy (CRM). By combining CRM with SMPAF, we can locate melanin reliably. However, we have the added benefit of eliminating background interference from other components inside mouse hair and skin. The melanin SMPAF signal from the mouse hair is a mixture of a two-photon process and a third-order process. The melanin SMPAF emission spectrum is activated by a 1505.9-nm laser light, and the resulting spectrum has a peak at 960 nm. The discovery of the emission peak may lead to a more energy-efficient method of background-free melanin detection with less photo-bleaching.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Zhenhua Lai ; Josef Kerimo ; Yair Mega and Charles A. DiMarzio
"Stepwise multiphoton activation fluorescence reveals a new method of melanin detection", J. Biomed. Opt. 18(6), 061225 (Jan 07, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.6.061225


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