Research Papers: Imaging

Single-cell photoacoustic thermometry

[+] Author Affiliations
Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri, 63130

J. Biomed. Opt. 18(2), 026003 (Feb 01, 2013). doi:10.1117/1.JBO.18.2.026003
History: Received July 30, 2012; Revised December 5, 2012; Accepted January 2, 2013
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Abstract.  A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Liang Gao ; Lidai Wang ; Chiye Li ; Yan Liu ; Haixin Ke, et al.
"Single-cell photoacoustic thermometry", J. Biomed. Opt. 18(2), 026003 (Feb 01, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.026003


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