Research Papers: Sensing

In vivo detection of epileptic brain tissue using static fluorescence and diffuse reflectance spectroscopy

[+] Author Affiliations
Nitin Yadav

Florida International University, Department of Biomedical Engineering, Miami, Florida

Sanjiv Bhatia, John Ragheb, Prasanna Jayakar

The Brain Institute, Miami Children’s Hospital, Miami, Florida

Rupal Mehta, William Yong

University of California at Los Angeles, Department of Pathology, Los Angeles, California

Wei-Chiang Lin

Florida International University, Department of Biomedical Engineering, Miami, Florida

The Brain Institute, Miami Children’s Hospital, Miami, Florida

J. Biomed. Opt. 18(2), 027006 (Feb 05, 2013). doi:10.1117/1.JBO.18.2.027006
History: Received August 30, 2012; Revised December 5, 2012; Accepted January 17, 2013
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Abstract.  Diffuse reflectance and fluorescence spectroscopy are used to detect histopathological abnormalities of an epileptic brain in a human subject study. Static diffuse reflectance and fluorescence spectra are acquired from normal and epileptic brain areas, defined by electrocorticography (ECoG), from pediatric patients undergoing epilepsy surgery. Biopsy specimens are taken from the investigated sites within an abnormal brain. Spectral analysis reveals significant differences in diffuse reflectance spectra and the ratio of fluorescence and diffuse reflectance spectra from normal and epileptic brain areas defined by ECoG and histology. Using these spectral differences, tissue classification models with accuracy above 80% are developed based on linear discriminant analysis. The differences between the diffuse reflectance spectra from the normal and epileptic brain areas observed in this study are attributed to alterations in the static hemodynamic characteristics of an epileptic brain, suggesting a unique association between the histopathological and the hemodynamic abnormalities in an epileptic brain.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Nitin Yadav ; Sanjiv Bhatia ; John Ragheb ; Rupal Mehta ; Prasanna Jayakar, et al.
"In vivo detection of epileptic brain tissue using static fluorescence and diffuse reflectance spectroscopy", J. Biomed. Opt. 18(2), 027006 (Feb 05, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.027006


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