Research Papers: Imaging

Blood optical clearing studied by optical coherence tomography

[+] Author Affiliations
Olga Zhernovaya

University of Limerick, Department of Physics and Energy, National Biophotonics and Imaging Platform, Tissue Optics and Microcirculation Imaging Facility, Ireland, Ireland

Saratov State University, International Research-Educational Center of Optical Technologies for Industry and Medicine “Photonics,” Russia

Valery V. Tuchin

Saratov State University, International Research-Educational Center of Optical Technologies for Industry and Medicine “Photonics,” Russia

Institute of Precise Mechanics and Control, RAS, Russia

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, Oulu, Finland

Martin J. Leahy

University of Limerick, Department of Physics and Energy, National Biophotonics and Imaging Platform, Tissue Optics and Microcirculation Imaging Facility, Ireland, Ireland

NUI Galway, School of Physics, National Biophotonics and Imaging Platform, Ireland, Tissue Optics and Microcirculation Imaging Facility, Ireland

Royal College of Surgeons, Dublin, Ireland

J. Biomed. Opt. 18(2), 026014 (Feb 06, 2013). doi:10.1117/1.JBO.18.2.026014
History: Received October 29, 2012; Revised January 8, 2013; Accepted January 22, 2013
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Abstract.  The main limitation of optical imaging techniques for studying biological tissues is light scattering leading to decreasing of transmittance, which lowers the imaging quality. In this case, an immersion method for optical clearing of biological tissues can provide a possible solution to this problem, because the application of biocompatible clearing agents can reduce light scattering. Optical clearing represents a promising approach to increasing the imaging depth for various techniques, for example, various spectroscopy and fluorescent methods, and optical coherence tomography (OCT). We investigate the improvement of light penetration depth in blood after application of polyethylene glycol, polypropylene glycol, propylene glycol, and hemoglobin solutions using an OCT system. Influence of clearing agents on light transport in tissues and blood was also investigated in the mouse tail vein.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Olga Zhernovaya ; Valery V. Tuchin and Martin J. Leahy
"Blood optical clearing studied by optical coherence tomography", J. Biomed. Opt. 18(2), 026014 (Feb 06, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.026014


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