Research Papers: Imaging

Novel registration for microcomputed tomography and bioluminescence imaging based on iterated optimal projection

[+] Author Affiliations
Xibo Ma, Zhenwen Xue, Chenghu Qin, Xin Yang, Jie Tian

Chinese Academy of Sciences, Intelligent Medical Research Center, Institute of Automation, Beijing 100190, China

Kexin Deng, Shouping Zhu

Xidian University, Life Sciences Research Center, School of Life Sciences and Technology, Xi’an, Shaanxi 710071, China

Xueyan Liu

Northeastern University, Sino-Dutch Biomedical and Information Engineering School, Shenyang 110819, China

J. Biomed. Opt. 18(2), 026013 (Feb 06, 2013). doi:10.1117/1.JBO.18.2.026013
History: Received July 6, 2012; Revised December 24, 2012; Accepted January 4, 2013
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Abstract.  As a high-sensitivity imaging modality, bioluminescence tomography can reconstruct the three-dimensional (3-D) location of an internal luminescent source based on the 3-D surface light distribution. However, we can only get the multi-orientation two-dimensional (2-D) bioluminescence distribution in the experiments. Therefore, developing an accurate universal registration method is essential for following bioluminescent source reconstruction. We can then map the multi-orientation 2-D bioluminescence distribution to the 3-D surface derived from anatomical information with it. We propose a 2-D -to-3-D registration method based on iterated optimal projection and applied it in a registration and reconstruction study of three transgenic mice. Compared with traditional registration methods based on the fixed points, our method was independent of the markers and the registration accuracy of the three experiments was improved by 0.3, 0.5, and 0.4 pixels, respectively. In addition, based on the above two registration results using the two registration methods, we reconstructed the 3-D location of the inner bioluminescent source in the three transgenic mice. The reconstruction results showed that the average error distance between the center of the reconstructed element and the center of the real element were reduced by 0.32, 0.48, and 0.39 mm, respectively.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Xibo Ma ; Kexin Deng ; Zhenwen Xue ; Xueyan Liu ; Shouping Zhu, et al.
"Novel registration for microcomputed tomography and bioluminescence imaging based on iterated optimal projection", J. Biomed. Opt. 18(2), 026013 (Feb 06, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.026013


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