Research Papers: Imaging

Noninvasive imaging of the early effect of sodium iodate toxicity in a rat model of outer retina degeneration with spectral domain optical coherence tomography

[+] Author Affiliations
Sepideh Hariri, Man Chun Tam, Donghyun Lee, Alireza Akhlagh Moayed, Kostadinka Bizheva

University of Waterloo, Department of Physics and Astronomy, Waterloo, Ontario, Canada

Denise Hileeto

University of Waterloo, School of Optometry and Vision Science, Waterloo, Ontario, Canada

J. Biomed. Opt. 18(2), 026017 (Feb 07, 2013). doi:10.1117/1.JBO.18.2.026017
History: Received September 6, 2012; Revised December 20, 2012; Accepted January 2, 2013
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Abstract.  An ultrahigh resolution spectral domain optical coherence tomography (SD-OCT) system is used to observe for the first time in vivo the early effect of sodium iodate (NaIO3) toxicity on retinal morphology. Retinal degeneration is induced in rats via tail vein injection of NaIO3 and structural changes in the outer retina are assessed longitudinally at baseline and 1, 2, 3, 6, 8, and 10 h, and 12 post drug administration with OCT, H&E histology, and IgG immunochemistry. Disruption of the structural integrity and changes in the optical reflectivity of the photoreceptor inner (IS) and outer segment (OS) layers are observed as early as 1 h post NaIO3 injection. A new layer is observed in the OCT tomograms to form between the retinal pigmented epithelium and the photoreceptors OS a few hours post NaIO3 injection. The dynamics and the low optical reflectivity of this layer, as well as cell swelling and disruption of the blood-retina barrier observed in the histological and immunohistochemistry cross-sections suggest that the layer corresponds to temporary fluid accumulation in the retina. Results from this study demonstrate the effectiveness of OCT technology for monitoring dynamic changes in the retinal morphology and provide better understanding of the early stages of outer retina degeneration induced by NaIO3 toxicity.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Sepideh Hariri ; Man Chun Tam ; Donghyun Lee ; Denise Hileeto ; Alireza Akhlagh Moayed, et al.
"Noninvasive imaging of the early effect of sodium iodate toxicity in a rat model of outer retina degeneration with spectral domain optical coherence tomography", J. Biomed. Opt. 18(2), 026017 (Feb 07, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.026017


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