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Research Papers: Imaging

Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo

[+] Author Affiliations
Yuri Dancik, Michael S. Roberts

University of Queensland, School of Medicine, Brisbane, Queensland 4102, Australia

University of South Australia, School of Pharmacy and Biomedical Sciences, Adelaide, South Australia 5000, Australia

Amandine Favre

University of Queensland, School of Medicine, Brisbane, Queensland 4102, Australia

Chong Jin Loy

Johnson & Johnson Asia Pacific, Singapore Research Center, Singapore 038985, Singapore

Andrei V. Zvyagin

Macquarie University, MQ Biofocus Research Centre, Sydney, New South Wales 2109, Australia

J. Biomed. Opt. 18(2), 026022 (Feb 14, 2013). doi:10.1117/1.JBO.18.2.026022
History: Received July 14, 2012; Revised December 20, 2012; Accepted January 29, 2013
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Abstract.  There is a growing body of literature showing the usefulness of multiphoton tomography (MPT) and fluorescence lifetime imaging for in situ characterization of skin constituents and the ensuing development of noninvasive diagnostic tools against skin diseases. Melanin and pigmentation-associated skin cancers constitute some of the major applications. We show that MPT and fluorescence lifetime imaging can be used to measure changes in cutaneous melanin concentration and that these can be related to the visible skin color. Melanin in the skin of African, Indian, Caucasian, and Asian volunteers is detected on the basis of its emission wavelength and fluorescence lifetimes in solution and in a melanocyte-keratinocyte cell culture. Fluorescence intensity is used to characterize the melanin content and distribution as a function of skin type and depth into the skin (stratum granulosum and stratum basale). The measured fluorescence intensities in given skin types agree with melanin amounts reported by others using biopsies. Our results suggest that spatial distribution of melanin in skin can be studied using MPT and fluorescence lifetime imaging, but further studies are needed to ascertain that the method can resolve melanin amount in smaller depth intervals.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Yuri Dancik ; Amandine Favre ; Chong Jin Loy ; Andrei V. Zvyagin and Michael S. Roberts
"Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo", J. Biomed. Opt. 18(2), 026022 (Feb 14, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.026022


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