JBO Letters

Discrimination of dimethyl sulphoxide diffusion coefficient in the process of optical clearing by confocal micro-Raman spectroscopy

[+] Author Affiliations
Ping Liu, Zhouyi Guo, Zhengfei Zhuang, Songhao Liu

South China Normal University, College of Biophotonics, MOE Key Laboratory of Laser Life Science, Guangzhou 510631, Guangdong, China

Yanyue Huang

South China Normal University, Laboratory of Photonic Information Technology, Guangzhou 510631, Guangdong, China

Jianping Wang

Guangdong Sensing Optics Science & Technology Company Limited, Foshan 528200, Guangdong, China

J. Biomed. Opt. 18(2), 020507 (Feb 15, 2013). doi:10.1117/1.JBO.18.2.020507
History: Received November 15, 2012; Revised December 21, 2012; Accepted January 2, 2013
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Abstract

Abstract.  Confocal micro-Raman spectroscopy is employed to study the diffusion process of dimethyl sulfoxide (DMSO) in porcine skin optical clearing. The variation of DMSO concentration with time at different depths of the skin was obtained and then the DMSO diffusion coefficient with the passive diffusion model was calculated. Results show that it has a significant difference at different depths of the skin. Also, the DMSO concentration with the depth at different times was obtained and the same method was used to find the change law of the DMSO diffusion coefficient. Results indicate that it also changes with the treatment time. The experimental results are consistent with the theoretical model in a previous study. The current results demonstrate that Raman spectroscopy has the ability to quantitatively monitor the process of optical clearing.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Ping Liu ; Yanyue Huang ; Zhouyi Guo ; Jianping Wang ; Zhengfei Zhuang, et al.
"Discrimination of dimethyl sulphoxide diffusion coefficient in the process of optical clearing by confocal micro-Raman spectroscopy", J. Biomed. Opt. 18(2), 020507 (Feb 15, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.2.020507


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