JBO Letters

Reflection-mode multifocal optical-resolution photoacoustic microscopy

[+] Author Affiliations
Guo Li, Konstantin I. Maslov, Lihong V. Wang

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 18(3), 030501 (Feb 27, 2013). doi:10.1117/1.JBO.18.3.030501
History: Received December 4, 2012; Revised February 11, 2013; Accepted February 12, 2013
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Abstract.  Compared with single-focus optical-resolution photoacoustic microscopy (OR-PAM), multifocal OR-PAM utilizes both multifocal optical illumination and an ultrasonic array transducer, significantly increasing the imaging speed. A reflection-mode multifocal OR-PAM system based on a microlens array that provides multiple foci as well as an ultrasonic array transducer that receives the excited photoacoustic waves from all foci simultaneously is presented. Using a customized microprism to reflect the incident laser beam to the microlens array, the multiple optical foci are aligned confocally with the focal zone of the ultrasonic array transducer. Experiments show the reflection-mode multifocal OR-PAM is capable of imaging microvessels in vivo, and it can image a 6×5×2.5mm3 volume at 16 μm lateral resolution in 2.5min, which was limited by the signal multiplexing ratio and laser pulse repetition rate.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Guo Li ; Konstantin I. Maslov and Lihong V. Wang
"Reflection-mode multifocal optical-resolution photoacoustic microscopy", J. Biomed. Opt. 18(3), 030501 (Feb 27, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.3.030501


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