Special Section on Optical Methods of Imaging in the Skin

In vivo detection of basal cell carcinoma: comparison of a reflectance confocal microscope and a multiphoton tomograph

[+] Author Affiliations
Martina Ulrich, Marisa Klemp, Maxim E. Darvin, Jürgen Lademann, Martina C. Meinke

Charité—Universitätsmedizin Berlin, Department of Dermatology, Allergology and Venerology, Charitéplatz 1, 10117 Berlin, Germany

Karsten König

JenLab GmbH, Schillerstr. 1, 07745 Jena, Germany

J. Biomed. Opt. 18(6), 061229 (Mar 01, 2013). doi:10.1117/1.JBO.18.6.061229
History: Received September 26, 2012; Revised January 25, 2013; Accepted January 29, 2013
Text Size: A A A

Abstract.  The standard diagnostic procedure for basal cell carcinoma (BCC) is invasive tissue biopsy with time-consuming histological examination. To reduce the number of biopsies, noninvasive optical methods have been developed providing high-resolution skin examination. We present direct comparison of a reflectance confocal microscope (RLSM) and a multiphoton tomograph (MPT) for BCC diagnosis. Both systems are applied to nine patients prior to surgery, and the results are analyzed, including histological results. Both systems prove suitable for detecting typical characteristics of BCC in various stages. The RLSM allows large horizontal overview images to be obtained, enabling the investigator to find the regions of interest quickly, e.g., BCC nests. Elongated cells and palisading structures are easily recognized using both methods. Due to the higher resolution, changes in nucleus diameter or cytoplasm could be visualized with the MPT. Therefore, the nucleus diameter, nucleus/cytoplasm ratio, and cell density are estimated for normal and BCC cells using the MPT. The nucleus of elongated BCC cells is significantly longer than other measured normal skin cells, whereas the cell density and nucleus/cytoplasm ratio of BCC cannot be significantly distinguished from granular cells.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Martina Ulrich ; Marisa Klemp ; Maxim E. Darvin ; Karsten König ; Jürgen Lademann, et al.
"In vivo detection of basal cell carcinoma: comparison of a reflectance confocal microscope and a multiphoton tomograph", J. Biomed. Opt. 18(6), 061229 (Mar 01, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.6.061229


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.