Research Papers: General

Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies

[+] Author Affiliations
Erden Ertorer

University of Western Ontario (Western University), Biomedical Engineering Program, 1151 Richmond Street, London, Ontario N6A 3K7, Canada

University of Western Ontario (Western University), Department of Physics and Astronomy, 1151 Richmond Street, London, Ontario N6A 3K7, Canada

Fartash Vasefi, Mohamadreza Najiminaini, Jeffrey J. L. Carson

Lawson Health Research Institute, Imaging Program, 750 Base Line Road, London, Ontario N6C 2R5, Canada

University of Western Ontario (Western University), Department of Medical Biophysics, Schulich School of Medicine and Dentistry, 1151 Richmond Street, London, Ontario N6A 3K7, Canada

Joel Keshwah, Douglas W. Hamilton

University of Western Ontario (Western University), Division of Oral Biology, Schulich School of Medicine and Dentistry, 1151 Richmond Street, London, Ontario N6A 3K7, Canada

Christopher Halfpap, Uwe Langbein

RheinMain University of Applied Sciences, Department of Physics, Am Brückweg 26, 6428 Rüsselsheim, Germany

Silvia Mittler

University of Western Ontario (Western University), Department of Physics and Astronomy, 1151 Richmond Street, London, Ontario N6A 3K7, Canada

J. Biomed. Opt. 18(3), 035002 (Mar 04, 2013). doi:10.1117/1.JBO.18.3.035002
History: Received September 27, 2012; Revised February 13, 2013; Accepted February 14, 2013
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Abstract.  The fabrication details to form large area systematically changing multishape nanoscale structures on a chip by laser interference lithography (LIL) are described. The feasibility of fabricating different geometries including dots, ellipses, holes, and elliptical holes in both x- and y- directions on a single substrate is shown by implementing a Lloyd’s interferometer. The fabricated structures at different substrate positions with respect to exposure time, exposure angle and associated light intensity profile are analyzed. Experimental details related to the fabrication of symmetric and biaxial periodic nanostructures on photoresist, silicon surfaces, and ion milled glass substrates are presented. Primary rat calvarial osteoblasts were grown on ion-milled glass substrates with nanotopography with a periodicity of 1200 nm. Fluorescent microscopy revealed that cells formed adhesions sites coincident with the nanotopography after 24 h of growth on the substrates. The results suggest that laser LIL is an easy and inexpensive method to fabricate systematically changing nanostructures for cell adhesion studies. The effect of the different periodicities and transition structures can be studied on a single substrate to reduce the number of samples significantly.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Erden Ertorer ; Fartash Vasefi ; Joel Keshwah ; Mohamadreza Najiminaini ; Christopher Halfpap, et al.
"Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies", J. Biomed. Opt. 18(3), 035002 (Mar 04, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.3.035002


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