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Research Papers: Sensing

Perfusion assessment in rat spinal cord tissue using photoplethysmography and laser Doppler flux measurements

[+] Author Affiliations
Justin P. Phillips

City University London, School of Engineering and Mathematical Sciences, Northampton Square, London, United Kingdom

Vincent Cibert-Goton, Peter J. Shortland

Queen Mary University of London, Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, United Kingdom

Richard M. Langford

Queen Mary University of London, Pain and Anaesthesia Research Centre London, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, London, United Kingdom

J. Biomed. Opt. 18(3), 037005 (Mar 11, 2013). doi:10.1117/1.JBO.18.3.037005
History: Received November 16, 2012; Revised January 21, 2013; Accepted February 18, 2013
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Abstract.  Animal models are widely used to investigate the pathological mechanisms of spinal cord injury (SCI), most commonly in rats. It is well known that compromised blood flow caused by mechanical disruption of the vasculature can produce irreversible damage and cell death in hypoperfused tissue regions and spinal cord tissue is particularly susceptible to such damage. A fiberoptic photoplethysmography (PPG) probe and instrumentation system were used to investigate the practical considerations of making measurements from rat spinal cord and to assess its suitability for use in SCI models. Experiments to assess the regional perfusion of exposed spinal cord in anesthetized adult rats using both PPG and laser Doppler flowmetry (LDF) were performed. It was found that signals could be obtained reliably from all subjects, although considerable intersite and intersubject variability was seen in the PPG signal amplitude compared to LDF. We present results from 30 measurements in five subjects, the two methods are compared, and practical application to SCI animal models is discussed.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Justin P. Phillips ; Vincent Cibert-Goton ; Richard M. Langford and Peter J. Shortland
"Perfusion assessment in rat spinal cord tissue using photoplethysmography and laser Doppler flux measurements", J. Biomed. Opt. 18(3), 037005 (Mar 11, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.3.037005


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