Research Papers: Therapeutic

Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats

[+] Author Affiliations
Kelly Rossetti Fernandes, Daniel Araki Ribeiro, Ana Claudia Muniz Rennó

Federal University of São Paulo, Department of Bioscience, Av. Ana Costa, 95, Vila Mathias, Santos, Sao Paulo, 11050-240 Brazil

Natália Camargo Rodrigues, Carla Tim, Anderson Amaro Santos, Nivaldo Antônio Parizotto, Patrícia Driusso

Federal University of São Carlos, Department of Physiotherapy, Rod Washington Luis, Km 235, Monjolinho, São Carlos, São Paulo, 13565-902 Brazil.

Heloisa Selistre de Araujo

Federal University of São Carlos, Department of Physiological Sciences, Rod Washington Luis, Km 235, Monjolinho, São Carlos, São Paulo, 13565-902 Brazil

J. Biomed. Opt. 18(3), 038002 (Mar 20, 2013). doi:10.1117/1.JBO.18.3.038002
History: Received August 29, 2012; Revised January 17, 2013; Accepted February 14, 2013
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Abstract.  We evaluate the effects of low-level laser therapy (LLLT) on the histological modifications and temporal osteogenic genes expression during the initial phase of bone healing in a model of bone defect in rats. Sixty-four Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 0.028cm2, 1.071W/cm2, 1 min and 34 s, 2.8 Joules, 100J/cm2) was performed for 1, 2, 3, and 5 sessions. Histopathology revealed that treated animals presented higher inflammatory cells recruitment, especially 12 and 36 h postsurgery. Also, a better tissue organization at the site of the injury, with the presence of granulation tissue and new bone formation was observed on days three and five postsurgery in the treated animals. The quantitative real time polymerase chain reaction showed that LLLT produced a significantly increase in mRNA expression of Runx-2, 12 h and three days post-surgery, a significant upregulation of alkaline phosphatase mRNA expression after 36 h and three days post-surgery and a significant increase of osteocalcin mRNA expression after three and five days. We concluded that LLLT modulated the inflammatory process and accelerated bone repair, and this advanced repair pattern in the laser-treated groups may be related to the higher mRNA expression of genes presented by these animals.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Kelly Rossetti Fernandes ; Daniel Araki Ribeiro ; Natália Camargo Rodrigues ; Carla Tim ; Anderson Amaro Santos, et al.
"Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats", J. Biomed. Opt. 18(3), 038002 (Mar 20, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.3.038002


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