Research Papers: Imaging

Spectroscopic imaging of serum proteins using quantum cascade lasers

[+] Author Affiliations
Anadi Mukherjee, Quentin Bylund, Manu Prasanna, Yotam Margalit

Infrasign LLC, 1440 Stone Pine Terrace #118, Fremont, California 94536

Tarik Tihan

Infrasign LLC, 1440 Stone Pine Terrace #118, Fremont, California 94536

bUniversity of California, Department of Pathology, Neuropathology Unit, 505 Parnassus Ave, San Francisco, California 94143

J. Biomed. Opt. 18(3), 036011 (Mar 20, 2013). doi:10.1117/1.JBO.18.3.036011
History: Received January 17, 2013; Revised February 15, 2013; Accepted February 28, 2013
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Abstract.  First measurements of biomedical imaging using quantum cascade lasers (QCL) are presented. We report spectroscopic imaging of serum proteins using QCLs as an example for monitoring surface biocontamination. We found that dry smears of human serum can be spectroscopically imaged, identified, and quantified with high sensitivity and specificity. The core parts of the imaging platform consist of optically multiplexing three QCLs and an uncooled microbolometer camera. We show imaging of human serum proteins at 6.1, 9.25, and 9.5 μm QCLs with high sensitivity and specificity. The sensitivity limit of 3μg/cm2 of the human serum spot was measured at an S/N=3.The specificity of human serum detection was measured at 99% probability at a threshold of 77μg/cm2. We anticipate our imaging technique to be a starting point for more sophisticated biomolecular diagnostic applications.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Anadi Mukherjee ; Quentin Bylund ; Manu Prasanna ; Yotam Margalit and Tarik Tihan
"Spectroscopic imaging of serum proteins using quantum cascade lasers", J. Biomed. Opt. 18(3), 036011 (Mar 20, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.3.036011


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