JBO Letters

Three-dimensional deconvolution microfluidic microscopy using a tilted channel

[+] Author Affiliations
Nicolas C. Pégard, Jason W. Fleischer

Princeton University, Department of Electrical Engineering, Olden Street, Princeton, New Jersey 08544

J. Biomed. Opt. 18(4), 040503 (Apr 03, 2013). doi:10.1117/1.JBO.18.4.040503
History: Received November 26, 2012; Revised March 1, 2013; Accepted March 8, 2013
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Abstract.  We have developed a microfluidic device that enables the computation of three-dimensional (3-D) images of flowing samples. Using a microfluidic channel that is tilted along the optical axis, we record several progressively defocused images of the flowing sample as it passes across the focal plane. The resulting focal stack is then deconvolved to generate 3-D images. Experimental results on flowing yeast cells reveal both volume and surface profile information. The microfluidic channel eliminates the need for a precise translation stage to control defocusing and enables high sample throughput in an insulated, nontoxic, liquid environment. The experimental device can be implemented in all existing microscopes as a modified slide stage and is ideally suited for 3-D profiling in flow cytometers.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Nicolas C. Pégard and Jason W. Fleischer
"Three-dimensional deconvolution microfluidic microscopy using a tilted channel", J. Biomed. Opt. 18(4), 040503 (Apr 03, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.4.040503


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