JBO Letters

350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus

[+] Author Affiliations
Jun Ki Kim

Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, Massachusetts 02114

Korea Advanced Institute of Science and Technology, WCU Graduate School of Nanoscience and Technology, Daejeon 305-701, Republic of Korea

Jin Woo Choi

Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, Massachusetts 02114

Wonkwang University, School of Dentistry, Department of Dental Pharmacology and Institute of Biomaterials·Implant, Iksan, Chonbuk 570-749, Republic of Korea

Seok Hyun Yun

Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, Massachusetts 02114

Korea Advanced Institute of Science and Technology, WCU Graduate School of Nanoscience and Technology, Daejeon 305-701, Republic of Korea

Harvard-MIT Health Sciences and Technology, Cambridge, Massachusetts 02140

J. Biomed. Opt. 18(5), 050502 (Apr 29, 2013). doi:10.1117/1.JBO.18.5.050502
History: Received January 29, 2013; Revised March 30, 2013; Accepted April 4, 2013
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Abstract.  Miniature endoscopic probes offer a solution for deep brain imaging by overcoming the limited depth of intravital microscopy. We describe a small-diameter (350 μm) graded-index optical probe with a side-view design for in vivo cellular imaging of the mammalian brain. The side-view probe provides a unique view of the vertical network of neurons and penetrating blood vessels. At a given insertion site, the translational and rotational scanning of the probe provides access to a large tissue area (>1mm2) across the cortex, hippocampus, thalamus, and hypothalamus.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Topics

Brain ; Endoscopes ; Tissues

Citation

Jun Ki Kim ; Jin Woo Choi and Seok Hyun Yun
"350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus", J. Biomed. Opt. 18(5), 050502 (Apr 29, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.5.050502


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