Research Papers: Sensing

Effects of pathology dyes on Raman bone spectra

[+] Author Affiliations
Karen A. Esmonde-White, Blake J. Roessler

University of Michigan Medical School, Department of Internal Medicine, Division of Rheumatology, 1150 W. Medical Center Drive, Ann Arbor, Michigan 48109-0688

Francis W. L. Esmonde-White, Michael D. Morris

University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109

J. Biomed. Opt. 18(5), 057002 (May 02, 2013). doi:10.1117/1.JBO.18.5.057002
History: Received December 20, 2012; Revised March 28, 2013; Accepted April 4, 2013
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Abstract.  We report an overlooked source of artifacts for clinical specimens, where unexpected and normally negligible contaminants can skew the interpretation of results. During an ongoing study of bone fragments from diabetic osteomyelitis, strong Raman signatures were found, which did not correspond with normal bone mineral or matrix. In a bone biopsy from the calcaneus of a patient affected by diabetic osteomyelitis, Raman microspectroscopic analysis revealed regions with both abnormal mineral and degraded collagen in addition to normal bone. Additional bands indicated a pathological material. Stenotrophomonas maltophilia was identified in the wound culture by independent microbiologic examination. We initially assigned the unusual bands to xanthomonadin, a bacterial pigment from S. maltophilia. However, the same bands were also found more than a year later on a second specimen that had been noticeably contaminated with pathology marking dye. Drop deposition/Raman spectroscopy of commonly used pathology dyes revealed that a blue tissue-marking dye was responsible for the unusual bands in both specimens, even in the first specimen where there was no visible evidence of contamination.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Karen A. Esmonde-White ; Francis W. L. Esmonde-White ; Michael D. Morris and Blake J. Roessler
"Effects of pathology dyes on Raman bone spectra", J. Biomed. Opt. 18(5), 057002 (May 02, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.5.057002


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