Mice of strain C57BL/6J (pigmented) were obtained from Jackson Laboratories (Bar Harbor, Maine) and Charles River (Wilmington, Massachusetts). All mouse imaging experiments were performed under protocols compliant to the Canadian Council on Animal Care, and with the approval of the University Animal Care Committee at Simon Fraser University. The mice were anesthetized [ketamine, xylazine, and acepromazine mixture ( body weight) injected intraperitoneally] and their pupils were dilated using a topical solution (atropine sulphate 1%) prior to imaging. Some motion was evident, however, due to animal respiration as observed during image acquisition. During imaging, the anesthetized mouse was placed in an adjustable holder with 5 deg of freedom that offered translation and rotation, allowing the mouse eye positioned for acquisition of images from different retinal eccentricities. In order to maintain corneal hydration, generous amounts of artificial tear gel (Novartis Tear-Gel, 10 g tube) were applied, and held in place with the fundus lens.