Research Papers: Imaging

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

[+] Author Affiliations
Hamideh Salehi, Elodie Middendorp, Ivan Panayotov, Pierre-Yves Collart Dutilleul, Frederic Cuisinier

Université Montpellier 1, Laboratoire Biologie-Santé Nanosciences, EA 4203, UFR Odontologie, 34193 Montpellier, France

Attila-Gergely Vegh

Université Montpellier 1, Laboratoire Biologie-Santé Nanosciences, EA 4203, UFR Odontologie, 34193 Montpellier, France

Institute of Biophysics, Hungarian Academy of Sciences, Biological Research Centre, 6726 Szeged, Hungary

Sathish Ramakrishnan, Csilla Gergely

Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, 34095 Montpellier, France

CNRS, Laboratoire Charles Coulomb UMR 5221, 34095 Montpellier, France

J. Biomed. Opt. 18(5), 056010 (May 09, 2013). doi:10.1117/1.JBO.18.5.056010
History: Received January 14, 2013; Revised March 31, 2013; Accepted April 12, 2013
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Abstract.  Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cytoplasm, indicating cell apoptosis via a caspase independent pathway.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Hamideh Salehi ; Elodie Middendorp ; Ivan Panayotov ; Pierre-Yves Collart Dutilleul ; Attila-Gergely Vegh, et al.
"Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel", J. Biomed. Opt. 18(5), 056010 (May 09, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.5.056010


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