Research Papers: Sensing

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating

[+] Author Affiliations
Alessandro Candiani, Sara Giannetti, Annamaria Cucinotta, Stefano Selleri

University of Parma, Department of Information Engineering, Area Parco delle Scienze 181/A, 43124, Parma, Italy

Alessandro Bertucci, Alex Manicardi, Roberto Corradini

University of Parma, Department of Chemistry, Area Parco delle Scienze 17/A, 43124 Parma, Italy

Maria Konstantaki, Stavros Pissadakis

Foundation for Research and Technology—Hellas FORTH, Institute of Electronic Structure and Laser IESL, Vassilika Vouton, 700 13 Heraklion, Crete, Greece

J. Biomed. Opt. 18(5), 057004 (May 10, 2013). doi:10.1117/1.JBO.18.5.057004
History: Received December 28, 2012; Revised April 5, 2013; Accepted April 12, 2013
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Abstract.  We describe a novel sensing approach based on a functionalized microstructured optical fiber-Bragg grating for specific DNA target sequences detection. The inner surface of a microstructured fiber, where a Bragg grating was previously inscribed, has been functionalized by covalent linking of a peptide nucleic acid probe targeting a DNA sequence bearing a single point mutation implicated in cystic fibrosis (CF) disease. A solution of an oligonucleotide (ON) corresponding to a tract of the CF gene containing the mutated DNA has been infiltrated inside the fiber capillaries and allowed to hybridize to the fiber surface according to the Watson-Crick pairing. In order to achieve signal amplification, ON-functionalized gold nanoparticles were then infiltrated and used in a sandwich-like assay. Experimental measurements show a clear shift of the reflected high order mode of a Bragg grating for a 100 nM DNA solution, and fluorescence measurements have confirmed the successful hybridization. Several experiments have been carried out on the same fiber using the identical concentration, showing the same modulation trend, suggesting the possibility of the reuse of the sensor. Measurements have also been made using a 100 nM mismatched DNA solution, containing a single nucleotide mutation and corresponding to the wild-type gene, and the results demonstrate the high selectivity of the sensor.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Alessandro Candiani ; Alessandro Bertucci ; Sara Giannetti ; Maria Konstantaki ; Alex Manicardi, et al.
"Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating", J. Biomed. Opt. 18(5), 057004 (May 10, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.5.057004


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