Research Papers: General

Single-walled carbon nanotubes as specific targeting and Raman spectroscopic agents for detection and discrimination of single human breast cancer cells

[+] Author Affiliations
Zeid A. Nima, Meena W. Mahmood, Alokita Karmakar, Thikra Mustafa, Shawn Bourdo, Yang Xu, Alexandru S. Biris

University of Arkansas at Little Rock, Center for Integrative Nanotechnology Sciences, Little Rock, Arkansas 72204

J. Biomed. Opt. 18(5), 055003 (May 14, 2013). doi:10.1117/1.JBO.18.5.055003
History: Received January 28, 2013; Revised April 8, 2013; Accepted April 22, 2013
Text Size: A A A

Abstract.  Raman active nano-complex agents based on single-walled carbon nanotubes (SWCNTs) are prepared and used for the swift and specific detection of breast cancer cells. SWCNTs are functionalized to bond covalently with the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody, which is specific to the highly expressed EpCAM antigen on the surface of breast cancer cells (MCF-7), but not on normal cells. The Raman nano-complexes demonstrate excellent ability to detect in vitro single breast cancer cells (MCF-7) and discriminate between them and normal fibroblast cells during the first 30 min of the targeting process. Raman linearity scanning is collected from a monolayer cell mixture, including both cancer cells and normal cells incubated with anti-EpCAM-SWCNTs, using a 633-nm laser excitation. The results shows that the Raman signal collected from targeted MCF-7 cells is extremely high, whereas there is little signal from the normal cells.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Zeid A. Nima ; Meena W. Mahmood ; Alokita Karmakar ; Thikra Mustafa ; Shawn Bourdo, et al.
"Single-walled carbon nanotubes as specific targeting and Raman spectroscopic agents for detection and discrimination of single human breast cancer cells", J. Biomed. Opt. 18(5), 055003 (May 14, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.5.055003


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.