JBO Letters

Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection

[+] Author Affiliations
Xiaoli Qi, Hanqing Xiong, Xiaohua Lv, Jianling Chen, Hui Gong, Qingming Luo, Shaoqun Zeng

Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Wuhan 430074, China

Huazhong University of Science and Technology, Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics of Ministry of Education, 1037 Luoyu Road, Wuhan 430074, China

J. Biomed. Opt. 18(5), 050506 (May 28, 2013). doi:10.1117/1.JBO.18.5.050506
History: Received March 8, 2013; Revised April 22, 2013; Accepted April 26, 2013
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Abstract.  A recently reported micro-optical sectioning tomography system has great potential to draw the neuronal circuits of large brain volume with submicron resolution by combining fine mechanic sectioning with simultaneous optical imaging. However, sectioning the fluorescence sample sometimes induces tears between adjacent tiles and causes difficulties in continuous fiber tracing from fluorescence imaging. A confocal detection to recover the interruptions of the nerve fiber is introduced. With a 50-μm-width confocal slit, the signal-to-background ratio is increased 16- to 49-fold more than that without the slit, which effectively improves the detectability of the signal in the interruptions and enables continuous tracing of the neuronal circuits.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Xiaoli Qi ; Hanqing Xiong ; Xiaohua Lv ; Jianling Chen ; Hui Gong, et al.
"Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection", J. Biomed. Opt. 18(5), 050506 (May 28, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.5.050506


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