The optical layout of the module is shown in Fig. 1. A mode-locked Ti:sapphire laser provides pulses of about 100 fs duration at a repetition rate of and a power of 1.7 W (Coherent Chameleon). For the results shown later, the laser spectrum was centered at 790 nm and had a FWHM of 9.8 nm. The intensity of the laser was controlled by the acousto-optic modulator (AOM) of the Zeiss LSM510 used in this work. To compensate for the group delay dispersion (GDD) of about introduced by the AOM and the other optical components up to the PCF, a prism compressor was introduced between the laser and the AOM. The laser can be routed directly to the microscope by the FM to enable its original functionality. For CARS, the FM is removed and the beam is expanded and collimated in BE1 to result in a Gaussian beam waist of , and a Rayleigh range of providing an acceptable beam divergence over the optical path of the setup. The half-wave plate HWP1 (WPA1212-2) rotates the linear polarization direction, which initially is in the plane of the setup. The beam is split by PBS1 (Thorlabs PBS102), with an intensity ratio adjusted by HWP1. The reflected beam is used as pump . The transmitted beam is directed by the piezoelectrically actuated mirror M1 and coupled in and out of the PCF (Newport FemtoWhite CARS) by oil immersion lenses (Nikon CFI Plan Achromat 50X). Using an immersion oil index-matched to fused silica (Cargille 06350) suppresses the surface reflection so that an optical isolator6 can be omitted. The PCF has two zero dispersion wavelengths around 775 and 945 nm and creates a continuum at 580 to 730 nm and 950 to 1150 nm with a well-defined chirp and low-intensity noise. The power coupled into the fiber was approximately 300 mW. The infrared branch of the continuum is used as Stokes beam . The visible branch of the continuum can be used as pulsed source for fluorescence lifetime imaging. For a pump of 790 nm wavelength, a vibrational wave number from 2000 to can be accessed, which can be extended down to using higher pump wavelengths. The Stokes is transmitted through an absorptive filter (3 mm of RG850 at the Brewster angle of 55 deg) to remove the light overlapping with the CARS spectral region. The intensity of the pump is adjusted by HWP2 and the polarizer P1, delayed by a retro-reflector R on a motorized linear stage, and recombined with the Stokes by a dichroic beam splitter (DBS) (CVI LWP-45-Rp-800-Tp-946-PW-1025-C). The pump pulse arrival time at relative to the Stokes (negative for pump leading) is adjusted by the linear stage, allowing one to change the IFD by a delay-scan. To set equal linear chirp of pump and Stokes for spectral focusing with adjustable pulse duration,3 glass blocks of known GVD (SF57) are inserted. The Stokes propagates through GB1 of thickness adjustable between 0 and 110 mm with 10 mm steps, and after recombination in the DBS pump and Stokes propagate through GB2 of thickness adjustable between 0 to 160 mm with 20 mm steps. Example spectra of the pump and Stokes beam are shown in Fig. 2 together with a nonresonant CARS spectrum generated for and by focusing the beam after GB3 through a high index ball lens and detected by a spectrometer integrated into the module.