Research Papers: Sensing

Discrimination of premalignant lesions and cancer tissues from normal gastric tissues using Raman spectroscopy

[+] Author Affiliations
Shuwen Luo, Changshui Chen

South China Normal University, Key Laboratory of Laser Life Science of Ministry of Education, Photon and Nano Research Centre for Biosciences, Guangzhou, Guangdong 510631, China

Hua Mao, Shaoqin Jin

Southern Medical University, Zhujiang Hospital, Department of Gastroenterology, Guangzhou, 510282 Guangdong, China

J. Biomed. Opt. 18(6), 067004 (Jun 04, 2013). doi:10.1117/1.JBO.18.6.067004
History: Received January 21, 2013; Revised April 25, 2013; Accepted April 29, 2013
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Abstract.  The feasibility of early detection of gastric cancer using near-infrared (NIR) Raman spectroscopy (RS) by distinguishing premalignant lesions (adenomatous polyp, n=27) and cancer tissues (adenocarcinoma, n=33) from normal gastric tissues (n=45) is evaluated. Significant differences in Raman spectra are observed among the normal, adenomatous polyp, and adenocarcinoma gastric tissues at 936, 1003, 1032, 1174, 1208, 1323, 1335, 1450, and 1655cm1. Diverse statistical methods are employed to develop effective diagnostic algorithms for classifying the Raman spectra of different types of ex vivo gastric tissues, including principal component analysis (PCA), linear discriminant analysis (LDA), and naive Bayesian classifier (NBC) techniques. Compared with PCA-LDA algorithms, PCA-NBC techniques together with leave-one-out, cross-validation method provide better discriminative results of normal, adenomatous polyp, and adenocarcinoma gastric tissues, resulting in superior sensitivities of 96.3%, 96.9%, and 96.9%, and specificities of 93%, 100%, and 95.2%, respectively. Therefore, NIR RS associated with multivariate statistical algorithms has the potential for early diagnosis of gastric premalignant lesions and cancer tissues in molecular level.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Shuwen Luo ; Changshui Chen ; Hua Mao and Shaoqin Jin
"Discrimination of premalignant lesions and cancer tissues from normal gastric tissues using Raman spectroscopy", J. Biomed. Opt. 18(6), 067004 (Jun 04, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.6.067004


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