JBO Letters

Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging

[+] Author Affiliations
Asael Papour, Oscar Stafsudd

University of California Los Angeles, Department of Electrical Engineering, Los Angeles, California 90095

Zach Taylor, Adria Sherman

University of California Los Angeles, Department of Bioengineering, 410 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, California 90095-1600

Desiree Sanchez, Gregory Lucey, William Yong

University of California Los Angeles, Brain Tumor Translational Resource, Los Angeles, California 90095

Linda Liau

University of California Los Angeles, Department of Neurosurgery, Los Angeles, California 90095

Warren Grundfest

University of California Los Angeles, Department of Electrical Engineering, Los Angeles, California 90095

University of California Los Angeles, Department of Bioengineering, 410 Westwood Plaza, Room 5121, Engineering V, P.O. Box 951600, Los Angeles, California 90095-1600

J. Biomed. Opt. 18(6), 060504 (Jun 12, 2013). doi:10.1117/1.JBO.18.6.060504
History: Received April 16, 2013; Revised May 7, 2013; Accepted May 10, 2013
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Abstract.  An autofluorescence lifetime wide-field imaging system that can generate contrast in underlying tissue structures of normal and malignant brain tissue samples with video rate acquisition and processing time is presented. Images of the investigated tissues were acquired with high resolution (35μm) using an algorithm to produce contrast based on differences in relative lifetimes. Sufficient contrast for delineation was produced without the computation of fluorescence decay times or Laguerre coefficients. The imaged tissues were sent for histological analysis that confirmed the detected imaged tissues morphological findings and correlations between relative lifetime maps and histology identified.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Asael Papour ; Zach Taylor ; Adria Sherman ; Desiree Sanchez ; Gregory Lucey, et al.
"Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging", J. Biomed. Opt. 18(6), 060504 (Jun 12, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.6.060504


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