Special Section on Advanced Biomedical Imaging and Sensing

Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor

[+] Author Affiliations
Ying-Feng Chang

Chang Gung University, Molecular Medicine Research Center, Taoyuan 33302, Taiwan

Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33302, Taiwan

Cheng-Han Chao

National Taiwan University Hospital Hsin-Chu Branch, Division of Nephrology, Department of Internal Medicine, Hsinchu 300, Taiwan

National Tsing Hua University, Institute of Molecular and Cellular Biology, Department of Life Science, Hsinchu 300, Taiwan

Lih-Yuan Lin

National Tsing Hua University, Institute of Molecular and Cellular Biology, Department of Life Science, Hsinchu 300, Taiwan

Cheng-Han Tsai

National Yang-Ming University, School of Biomedical Science and Engineering, Department of Biomedical Imaging and Radiological Sciences, Taipei 112, Taiwan

Chien Chou

Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33302, Taiwan

Chang Gung University, Health Aging Research Center, Taoyuan 33302, Taiwan

Yi-Jang Lee

National Yang-Ming University, School of Biomedical Science and Engineering, Department of Biomedical Imaging and Radiological Sciences, Taipei 112, Taiwan

National Yang-Ming University, Biophotonics & Molecular Imaging Research Center, Taipei 112, Taiwan

J. Biomed. Opt. 19(1), 011004 (Jun 20, 2013). doi:10.1117/1.JBO.19.1.011004
History: Received February 22, 2013; Revised May 14, 2013; Accepted May 24, 2013
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Abstract.  The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n=37 from 47 cases analyzed) was twofold higher than that of healthy adults (n=21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Ying-Feng Chang ; Cheng-Han Chao ; Lih-Yuan Lin ; Cheng-Han Tsai ; Chien Chou, et al.
"Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor", J. Biomed. Opt. 19(1), 011004 (Jun 20, 2013). ; http://dx.doi.org/10.1117/1.JBO.19.1.011004


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