Special Section on Optical Imaging, Sensing, and Light Interactions in Cells and Tissues

On-chip optical stimulation and electrical recording from cells

[+] Author Affiliations
Alexey Yakushenko, Vanessa Maybeck, Andreas Offenhäusser, Bernhard Wolfrum

Institute of Bioelectronics (PGI-8/ICS-8) and JARA-Fundamentals of Future Information Technology, Forschungszentrum Jülich, 52425 Jülich, Germany

Zheng Gong

University of Strathclyde, Institute of Photonics, G4 0NW Glasgow, United Kingdom

mLED Ltd, G1 1XN Glasgow, United Kingdom

Boris Hofmann

Institute of Bioelectronics (PGI-8/ICS-8) and JARA-Fundamentals of Future Information Technology, Forschungszentrum Jülich, 52425 Jülich, Germany

University of Tübingen, Institute of Applied Physics, 72076 Tübingen, Germany

Erdan Gu, Martin Dawson

University of Strathclyde, Institute of Photonics, G4 0NW Glasgow, United Kingdom

J. Biomed. Opt. 18(11), 111402 (Jun 20, 2013). doi:10.1117/1.JBO.18.11.111402
History: Received February 22, 2013; Revised April 10, 2013; Accepted April 12, 2013
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Abstract.  We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Alexey Yakushenko ; Zheng Gong ; Vanessa Maybeck ; Boris Hofmann ; Erdan Gu, et al.
"On-chip optical stimulation and electrical recording from cells", J. Biomed. Opt. 18(11), 111402 (Jun 20, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.11.111402


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