Research Papers: Therapeutic

Relationship between the chemical and morphological characteristics of human dentin after Er:YAG laser irradiation

[+] Author Affiliations
Luís Eduardo Silva Soares

Universidade do Vale do Paraíba, UNIVAP, Department of Dental Materials and Operative Dentistry, School of Dentistry, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil

Ovídio César Lavesa Martin, Airton Abrahão Martin

Universidade do Vale do Paraíba, UNIVAP, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil

Lilian Tan Moriyama, Cristina Kurachi

Universidade de São Paulo, USP, Instituto de Física de São Carlos, IFSC, Departamento de Física e Ciências dos Materiais, FCM, Av. Trabalhador São-Carlense, 400, 13566-590, São Carlos, São Paulo, Brazil

J. Biomed. Opt. 18(6), 068001 (Jun 24, 2013). doi:10.1117/1.JBO.18.6.068001
History: Received November 9, 2012; Revised May 28, 2013; Accepted June 3, 2013
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Abstract.  The effects of laser etching on dentin are studied by microenergy-dispersive x-ray fluorescence spectrometry (μ-EDXRF) and scanning electron microscopy (SEM) to establish the correlation of data obtained. Fifteen human third molars are prepared, baseline μ-EDXRF mappings are performed, and ten specimens are selected. Each specimen received four treatments: acid etching (control-CG) or erbium:yttrium–aluminum–garnet (Er:YAG) laser irradiation (I—100 mJ, II—160 mJ, and III—220 mJ), and maps are done again. The Ca and P content are significantly reduced after acid etching (p<0.0001) and increased after laser irradiation with 220 mJ (Ca: p<0.0153 and P: p=0.0005). The Ca/P ratio increased and decreased after CG (p=0.0052) and GI (p=0.0003) treatments, respectively. CG treatment resulted in lower inorganic content (GI: p<0.05, GII: p<0.01, and GIII: p<0.01) and higher Ca/P ratios than laser etching (GI: p<0.001, GII: p<0.01, and GIII: p<0.01). The SEM photomicrographies revealed open (CG) and partially open dentin tubules (GI, GII, and GIII). μ-EDXRF mappings illustrated that acid etching created homogeneous distribution of inorganic content over dentin. Er:YAG laser etching (220 mJ) produced irregular elemental distribution and changed the stoichiometric proportions of hydroxyapatite, as showed by an increase of mineral content. Decreases and increases of mineral content in the μ-EDXRF images are correlated to holes and mounds, respectively, as found in SEM images.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Luís Eduardo Silva Soares ; Ovídio César Lavesa Martin ; Lilian Tan Moriyama ; Cristina Kurachi and Airton Abrahão Martin
"Relationship between the chemical and morphological characteristics of human dentin after Er:YAG laser irradiation", J. Biomed. Opt. 18(6), 068001 (Jun 24, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.6.068001


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