JBO Letters

Combined spectrophotometry and tensile measurements of human connective tissues: potentials and limitations

[+] Author Affiliations
Markus Ernstberger, Tobias Baselt, Peter Hartmann

University of Applied Sciences, Applied Physics and Computer Science, Westsaechsische Hochschule Zwickau, 08056 Zwickau, Germany

Freddy Sichting

University of Leipzig, Institute of Anatomy, Faculty of Medicine, 04103 Leipzig, Germany

Chemnitz University of Technology, Institute of Sport Science, Department Human Locomotion, 09126 Chemnitz, Germany

Gabriela Aust

University of Leipzig, Department of Surgery, Research Laboratories, Faculty of Medicine, 04103 Leipzig, Germany

Niels Hammer

University of Leipzig, Institute of Anatomy, Faculty of Medicine, 04103 Leipzig, Germany

J. Biomed. Opt. 18(6), 060506 (Jun 24, 2013). doi:10.1117/1.JBO.18.6.060506
History: Received March 25, 2013; Revised May 16, 2013; Accepted May 20, 2013
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Abstract.  Strain-dependent transmission data of nine iliotibial tract specimens are determined using a custom-built optical setup with a halogen light source and an industrial norm material testing machine. Polarized light microscopy and hematoxylin-eosin staining indicated that lateral contraction of collagen structures is responsible for total intensity variations during a 20-cycle preconditioning and a 5-cycle tensile test. Tensile force progress is opposite to total transmission progress. Due to dehydration, wavelength-specific radiation intensity shifting is determined during the test, primarily noticeable in a water absorption band between 1400 and 1500 nm. The results show the capability of integrating spectrophotometry technology into biomechanics for determining structural alterations of human collagen due to applied strain. Being more sensitive to drying, spectrophotometry may likely serve as a quality control in stress-strain testing of biological structures.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Markus Ernstberger ; Freddy Sichting ; Tobias Baselt ; Peter Hartmann ; Gabriela Aust, et al.
"Combined spectrophotometry and tensile measurements of human connective tissues: potentials and limitations", J. Biomed. Opt. 18(6), 060506 (Jun 24, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.6.060506


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