After imaging, the neural cells were fixed for 20 min with a 4% paraformaldehyde solution (Biosesang, Seongnam, South Korea) and rinsed three times with PBS at room temperature. The fixed cells were treated for 1 h with a blocking solution containing 4% normal goat serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Sigma-Aldrich), 2% bovine serum albumin (Sigma-Aldrich), and 2% FBS (Sigma-Aldrich). After three 5-min PBS washes, rhodamine-conjugated phalloidin (Cytoskeleton Inc., Denver, Colorado) was applied to the cells for 1 h to stain the F-actin. For visualizing the microtubules in the cells, anti-tubulin beta3 (Millipore, Billerica Massachusetts) was applied to cells for 1 h, and then the cells were incubated in Alexa Fluor 594 (Invitrogen). After completion of the procedure for F-actin or tubulin staining, the cells were washed twice for 5 min with PBS, and then treated with DAPI (Molecular Probes, Eugene, Oregon) in PBS for 20 min to stain the nuclei. After the last 5-min PBS wash, the cells were mounted in fluorescent mounting medium (Dako Cytomation, Carpinteria, California), and then covered with a cover glass (Paul Marienfeld GmbH & Co. KG, Lauda-Königshofen, Germany).