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Special Section on Advanced Biomedical Imaging and Sensing

High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

[+] Author Affiliations
Kyoohyun Kim, HyeOk Yoon, YongKeun Park

Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon 305-701, Republic of Korea

Monica Diez-Silva, Ming Dao

Massachusetts Institute of Technology, Department of Material Science and Engineering, Cambridge, Massachusetts 02142

Ramachandra R. Dasari

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02194

J. Biomed. Opt. 19(1), 011005 (Jun 25, 2013). doi:10.1117/1.JBO.19.1.011005
History: Received February 23, 2013; Revised May 6, 2013; Accepted May 29, 2013
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Abstract.  We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Kyoohyun Kim ; HyeOk Yoon ; Monica Diez-Silva ; Ming Dao ; Ramachandra R. Dasari, et al.
"High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography", J. Biomed. Opt. 19(1), 011005 (Jun 25, 2013). ; http://dx.doi.org/10.1117/1.JBO.19.1.011005


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