Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics

Quantitative observation of focused-ultrasound-induced vascular leakage and deformation via fluorescein angiography and optical coherence tomography

[+] Author Affiliations
Meng-Tsan Tsai, Kung-Min Lin, Yu-Xiang Lin, Tzu-Han Lin, Ting-Chia Chang, Jiann-Der Lee, Hao-Li Liu

Chang Gung University, Department of Electrical Engineering, 259, Wen-Hwa 1st Road, Kwei-Shan, Taoyuan 33302, Taiwan

Cheng-Kuang Lee

Chang Gung University, Department of Electrical Engineering, 259, Wen-Hwa 1st Road, Kwei-Shan, Taoyuan 33302, Taiwan

Chang Gung University, Graduate Institute of Medical Mechatronics, 259, Wen-Hwa 1st Road, Kwei-Shan, Taoyuan 33302, Taiwan

J. Biomed. Opt. 18(10), 101307 (Jun 28, 2013). doi:10.1117/1.JBO.18.10.101307
History: Received February 27, 2013; Revised May 19, 2013; Accepted May 23, 2013
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Abstract.  Focused ultrasound (FUS) is a recently discovered noninvasive technique for local and temporal enhancement of vascular permeability, which facilitates drug delivery from the vessels into the surrounding tissue. However, exposure to FUS at a high intensity may cause permanent damage. To investigate the effects of the FUS treatment on blood vessels, we propose to use fluorescein angiography (FA) and optical coherence tomography (OCT) for real-time observation of the diffusion of fluorescence dye from blood vessels and to evaluate the morphological changes of the vessels in vivo. With time-resolved FA imaging, the relationship between the exposed power and the improved permeability of the vessels can be assessed according to the enhancement of the fluorescent intensity due to the dye leakage. Furthermore, the variation of the time-resolved fluorescent intensities can be used to identify the occurrence of dye leakage. In contrast, OCT can be implemented for the reconstruction of tissue microstructures. To quantitatively evaluate the morphological changes of the vessels after the FUS exposure with OCT, a new algorithm was proposed to estimate the vessel area based on the comparison of backscattering properties resulting from the tissue and vascular structures. Results showed that the vessel area increased as the exposed power increased, and the area became significantly larger at a higher FUS exposure power of 10 W. In conclusion, integrated FA and OCT observation can be potentially effective for monitoring the outcome and investigating the effects of FUS treatment.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Meng-Tsan Tsai ; Cheng-Kuang Lee ; Kung-Min Lin ; Yu-Xiang Lin ; Tzu-Han Lin, et al.
"Quantitative observation of focused-ultrasound-induced vascular leakage and deformation via fluorescein angiography and optical coherence tomography", J. Biomed. Opt. 18(10), 101307 (Jun 28, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.101307


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