Special Section on Advanced Biomedical Imaging and Sensing

Observation of the dynamic germination of single bacterial spores using rapid Raman imaging

[+] Author Affiliations
Lingbo Kong, Yong-qing Li

East Carolina University, Department of Physics, Greenville, North Carolina 27858-4353

Peter Setlow

University of Connecticut Health Center, Department of Molecular, Microbial and Structural Biology, Farmington, Connecticut 06030-3305

J. Biomed. Opt. 19(1), 011003 (Jul 10, 2013). doi:10.1117/1.JBO.19.1.011003
History: Received March 30, 2013; Revised May 13, 2013; Accepted May 15, 2013
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Abstract.  The dynamics of bacterial spore germination were successfully observed using a fast Raman imaging system, in combination with real-time phase contrast microscopy. By using a multifocus scan scheme, the spontaneous Raman-scattering imaging acquisition speed was increased to 30s per frame while maintaining diffraction-limited resolution, which allowed monitoring of the dynamics of spore germination on a time scale of tens of seconds to a few minutes. This allowed simultaneous gathering of rich spatial distribution information on different cellular components including time-lapse molecular images of Ca-dipicolinic acid, protein, and nucleic acid during germination of single bacterial spores for the periods of 30 to 60 min.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Lingbo Kong ; Peter Setlow and Yong-qing Li
"Observation of the dynamic germination of single bacterial spores using rapid Raman imaging", J. Biomed. Opt. 19(1), 011003 (Jul 10, 2013). ; http://dx.doi.org/10.1117/1.JBO.19.1.011003


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