Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics

Functional probe for annulus fibrosus-targeted intervertebral disc degeneration imaging

[+] Author Affiliations
Hye-Yeong Kim

University of Pittsburgh, Department of Radiology, School of Medicine, Molecular Imaging Laboratory, Pittsburgh, Pennsylvania 15219

Michael Mcclincy, Nam V. Vo, James D. Kang

University of Pittsburgh Medical Center, Department of Orthopedic Surgery, Ferguson Laboratory for Orthopaedic Research, Pittsburgh, Pennsylvania 15213

Gwendolyn A. Sowa

University of Pittsburgh Medical Center, Department of Orthopedic Surgery, Ferguson Laboratory for Orthopaedic Research, Pittsburgh, Pennsylvania 15213

University of Pittsburgh Medical Center, Department of Physical Medicine and Rehabilitation, Pittsburgh, Pennsylvania 15213

Mingfeng Bai

University of Pittsburgh, Department of Radiology, School of Medicine, Molecular Imaging Laboratory, Pittsburgh, Pennsylvania 15219

University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213

J. Biomed. Opt. 18(10), 101308 (Jul 09, 2013). doi:10.1117/1.JBO.18.10.101308
History: Received February 28, 2013; Revised May 9, 2013; Accepted June 3, 2013
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Abstract.  Intervertebral disc degeneration (IDD) is closely associated with low back pain. Typically nonsurgical treatment of IDD is the most effective when detected early. As such, establishing reliable imaging methods for the early diagnosis of disc degeneration is critical. The cellular and tissue localization of a facile functional fluorescent probe, HYK52, that labels disc annulus fibrosus is reported. HYK52 was synthesized with high yield and purity via a two-step chemical reaction. Rabbit disc cell studies and ex vivo tissue staining images indicated intracellular localization and intervertebral disc (IVD) tissue binding of HYK52 with negligible cytotoxicity. Moreover, HYK52 is purposefully designed with a functional terminal carboxyl group to allow for coupling with various signaling molecules for multimodal imaging applications. These results suggest that this IVD-targeted probe may have great potential in early diagnosis of IDD.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Hye-Yeong Kim ; Michael Mcclincy ; Nam V. Vo ; Gwendolyn A. Sowa ; James D. Kang, et al.
"Functional probe for annulus fibrosus-targeted intervertebral disc degeneration imaging", J. Biomed. Opt. 18(10), 101308 (Jul 09, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.101308


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