Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics

Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells

[+] Author Affiliations
Mark Golberg, Sharon Zlochiver

Tel Aviv University, Department of Biomedical Engineering, Tel-Aviv 69978, Israel

Dror Fixler, Yevgeny Beiderman

Bar-Ilan University, Faculty of Engineering, Ramat-Gan 52900, Israel

Asher Shainberg

Bar-Ilan University, The Mina and Everard Goodman Faculty of Life Sciences, Ramat-Gan 52900, Israel

Vicente Micó, Javier Garcia

Universitat de València, Departamento de Óptica, Calle Doctor Moliner 50, 46100 Burjassot, Spain

Zeev Zalevsky

Bar-Ilan University, Faculty of Engineering, Ramat-Gan 52900, Israel

Friedrich-Alexander Erlangen-Nuremberg University, Erlangen 91052, Germany

J. Biomed. Opt. 18(10), 101310 (Jul 15, 2013). doi:10.1117/1.JBO.18.10.101310
History: Received April 11, 2013; Revised May 31, 2013; Accepted June 12, 2013
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Abstract.  An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (1000 cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation of the effect of isoproteranol on cell contraction process.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Mark Golberg ; Dror Fixler ; Asher Shainberg ; Sharon Zlochiver ; Vicente Micó, et al.
"Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells", J. Biomed. Opt. 18(10), 101310 (Jul 15, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.101310


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